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Neovascularization occurs in response to crush lesions of adult frog
The appearance of these blood vessels is coincident with the appearance of granulocytes and macrophages in the nerve. Successful regeneration of the adult frog optic nerve may depend on this neovascularization to facilitate the rapid removal of cellular debris and to supply regenerating axons with trophic substances.
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Cellular reactions at the lesion site after crushing of the rat optic nerve
The crush injury produced a vast area of tissue damage including the disruption of the blood-brain barrier (BBB). In the first days after crushing, astrocytes were absent from the lesion site. S1OOP-positive astrocytes reappeared in the lesion center as early as 6 days after crushing.
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As cellular debris are cleared and lesion cores mature, they become composed primarily of these stromal non-neural cells intermingled with endothelial progenitors and newly formed blood vessels, as well as with extravasated blood-borne cells such as fibrocytes and diverse immune cells, including hematogenous macrophages, neutrophils, lymphocytes and other leukocytes (which change over time
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Optic Nerve Regeneration After Crush Remodels the Injury Site
IIR promotes a robust phagocytic response that improves clearance of myelin and axon debris. This growth-permissive molecular remodeling of the crush injury site extends our current understanding of IIR to include mechanisms extrinsic to the RGC.
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